Purification of Alkaline Proteinase from Aspergillus Candidus
نویسندگان
چکیده
منابع مشابه
Alkaline serine proteinase from Thermomonospora
The serine proteinase isolated from Thermomonospora fusca YX shows considerable thermal stability up to 80 °C, and progressive inactivation occurs at higher temperatures. Lyotropic salts affected the thermal stability of the enzyme at 85 °C, suggesting that disruption of hydrophobic interactions play an important role in the decreased thermal stability of the enzyme above 80 'C. Thermal stabili...
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A serine proteinase (Alp) from the culture supernate of a clinical isolate of Aspergillus fumigatus was purified to virtual homogeneity at a yield of 41%. The procedure involved affinity chromatography on agarose-epsilon-amino-caproyl-D-tryptophan methyl ester. Alp had an estimated mol. wt of 32 Kda and the pI was determined at pH 7.9. The enzyme was fully inhibited by phenylmethyl sulphonyl fl...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1972
ISSN: 0002-1369
DOI: 10.1080/00021369.1972.10860466